Tuberculosis is still one of the leading causes of death in the world. The COVID-19 pandemic increased the number of newly diagnosed cases, bringing it to 6.4 million in 2021, the number of deaths raised up to 1.4 million. An estimated 10.6 million people fell ill with TB worldwide in 2021, an increase of 4.5% from 10.1 million to 10.6 million was detected. Since the diagnosis of tuberculosis pathology can reach a sufficiently large amount of time, the task of rapid identification of both tuberculosis pathology and the antibiotic resistance of the mycobacterium becomes paramount. Methods based on real-time PCR are used as classical microbiological technologies, however, they have significant limitations in the number of antibiotics for determining resistance. As the vibration spectra (IR or Raman) of Mycobacterium tuberculosis cell is complex and it is difficult to find specific biomarkers for cell fast differentiation.
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