High-speed fluorescence excitation-scanning hyperspectral imaging microscopy using thin-film tunable filters

Santina Johnson; Naga Annamdevula; Thomas C. Rich; Chris Ballard; Silas J. Leavesley

doi:10.1117/12.3001600

12 March 2024 High-speed fluorescence excitation-scanning hyperspectral imaging microscopy using thin-film tunable filters

Santina Johnson, Naga Annamdevula, Thomas C. Rich, Chris Ballard, Silas J. Leavesley

Author Affiliations +

Proceedings Volume 12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; 128460B (2024) https://doi.org/10.1117/12.3001600
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Hyperspectral imaging (HSI) technologies have enabled a range of experimental techniques and studies in the fluorescence microscopy field. Unfortunately, a drawback of many HSI microscope platforms is increased acquisition time required to collect images across many spectral bands, as well as signal loss due to the need to filter or disperse emitted fluorescence into many discrete bands. We have previously demonstrated that an alternative approach of scanning the fluorescence excitation spectrum can greatly improve system efficiency by decreasing light losses associated with emission filtering. Our initial system was configured using an array of thin-film tunable filters (TFTFs, VersaChrome, Semrock) mounted in a tiltable filter wheel (VF-5, Sutter) that required ~150-200 ms to switch between wavelengths. Here, we present a new configuration for high-speed switching of TFTFs to allow rapid time-lapse HSI microscopy. A TFTF array was mounted in a custom holder that was attached to a piezoelectric rotation mount (ThorLabs), allowing high-speed rotation. Switching between adjacent filters was achieved using the internal optics of a DG-4 lightsource (Sutter Instrument), including a pair of off-axis parabolic mirrors and galvanometers. Output light was coupled to a liquid lightguide and into an inverted widefield fluorescence microscope (TI-2, Nikon Instruments). Initial tests indicate that the HSI system provides a 15-20 nm bandwidth tunable excitation band and ~10-20 ms wavelength switch time, allowing for high-speed HSI imaging of dynamic cellular events. This work was supported by NIH P01HL066299, R01HL169522, NIH TL1TR003106, and NSF MRI1725937.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Santina Johnson, Naga Annamdevula, Thomas C. Rich, Chris Ballard, and Silas J. Leavesley "High-speed fluorescence excitation-scanning hyperspectral imaging microscopy using thin-film tunable filters", Proc. SPIE 12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, 128460B (12 March 2024); https://doi.org/10.1117/12.3001600

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