Spectroscopic characterization of the interaction of Gentiana with bovine serum albumin

Chennan Li; Jiping Yuan; Lexin Wang

doi:10.1117/12.3016298

1 February 2024 Spectroscopic characterization of the interaction of Gentiana with bovine serum albumin

Chennan Li, Jiping Yuan, Lexin Wang

Proceedings Volume 13068, Fifth International Conference on Optoelectronic Science and Materials (ICOSM 2023); 1306811 (2024) https://doi.org/10.1117/12.3016298
Event: Fifth International Conference on Optoelectronic Science and Materials (ICOSM 2023), 2023, Hefei, China

Abstract

Fluorescence spectroscopy was used to explore the mechanism of action of Gentiana produced in Daqing with bovine serum albumin (BSA). The Stern-Volmer equation and burst constants at various temperatures (288 K, 298 K, 305 K, 310 K) indicated that the main burst mechanism of Gentiana and BSA was the dynamic burst caused by intermolecular collisions; The fluorescence intensity of BSA was analyzed by fluorescence spectroscopy to and showed different degrees of quenching with increasing concentration of Gentiana, but none of the fluorescence peak shapes were changed, indicating that the herbs interacted with BSA; On the basis of the binding site n<1 and the thermodynamic parameters ΔG <0; ΔH >0, ΔS >0, it is concluded that Gentiana is weakly able to bind to BSA and that the force between them is a hydrophobic force. The study elucidates the basic data on the mechanism of action of Gentiana with BSA, which will help the further development and application of Gentiana.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Chennan Li, Jiping Yuan, and Lexin Wang "Spectroscopic characterization of the interaction of Gentiana with bovine serum albumin", Proc. SPIE 13068, Fifth International Conference on Optoelectronic Science and Materials (ICOSM 2023), 1306811 (1 February 2024); https://doi.org/10.1117/12.3016298

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KEYWORDS

Quenching

Fluorescence

Molecular interactions

Medicine

Fluorescence spectroscopy

Molecules

Spectroscopy

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