Use of polarization to separate on-axis scattered and unscattered light in red blood cells

Dhiraj K. Sardar; Babak Nemati; Frederick J. Barrera

doi:10.1117/12.44124

1 June 1991 Use of polarization to separate on-axis scattered and unscattered light in red blood cells

Dhiraj K. Sardar, Babak Nemati, Frederick J. Barrera

Proceedings Volume 1427, Laser-Tissue Interaction II; (1991) https://doi.org/10.1117/12.44124
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The separation of on-axis scattered and unscattered transmission through turbid media has been a difficult experimental task in recent years. This study suggests the use of a polarimeter to filter out the contribution of scattered light to the net on-axis transmission. Red blood cells (RBC) were used to produce the scattering effect. The scattering level was varied by: (1) altering the distance of the detector from the sample, (2) using erythrocytes from three different species, e.g., the dog, goat, and human, which are know to have different RBC sizes, and (3) allowing the RBCs from each species to shrink and swell osmotically. An He-Ne laser was used as the source of the radiation so that data were obtained at a wavelength in the spectral region used in oximetry and hemoglobinometry. In each case, the difference in the scattering cross sections obtained for each sample, with and without polarization filtering, gave us a measure of the filtered scattered light. The results obtained were in close agreement with the expected contribution of scattered radiation to the net axial transmission. This method may be used effectively for all studies involving measurements of on-axis transmission through turbid media, such as biological tissue.

Citation Download Citation

Dhiraj K. Sardar, Babak Nemati, and Frederick J. Barrera "Use of polarization to separate on-axis scattered and unscattered light in red blood cells", Proc. SPIE 1427, Laser-Tissue Interaction II, (1 June 1991); https://doi.org/10.1117/12.44124

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