Flow injection analysis with bioluminescence-based fiber-optic biosensors

Loic J. Blum; Sabine Gautier; Pierre R. Coulet

doi:10.1117/12.47122

1 September 1991 Flow injection analysis with bioluminescence-based fiber-optic biosensors

Loic J. Blum, Sabine Gautier, Pierre R. Coulet

Proceedings Volume 1510, Chemical and Medical Sensors; (1991) https://doi.org/10.1117/12.47122
Event: ECO4 (The Hague '91), 1991, The Hague, Netherlands

Abstract

Fiber optic biosensors based on the firefly and the bacterial bioluminescence reactions have been constructed and incorporated in a specially designed flow-cell for the sensitive determination of ATP and NADH, respectively. The bioluminescence enzymes were immobilized on preactivated polyamide membranes which were placed in close contact with the surface on one end of a glass-fiber bundle, the other end being connected to the photomultiplier tube of a luminometer. When using the continuous-flow device with the firefly luciferase or the bacterial system immobilized separately on different membranes, the detection limit for ATP and NADH were 0.25 and 2 pmol, respectively. The versatility of the fiber optic probe has been improved by co-immobilizing the bacterial bioluminescent system and the firefly luciferase on the same support enabling the use of a single sensor for the selective, specific, and alternate determination of these two analytes. Compatible reaction conditions preserving the activity of each co-immobilized enzyme without impairing its stability were found. The selection of the appropriate reaction medium was done using a four port valve. Alternate quantification of ATP and NADH could then be performed in the linear ranges 0.25 pmol - 3 nmol and 5 pmol - 1 nmol, respectively with a RSD of 4.0 - 4.5%.

Citation Download Citation

Loic J. Blum, Sabine Gautier, and Pierre R. Coulet "Flow injection analysis with bioluminescence-based fiber-optic biosensors", Proc. SPIE 1510, Chemical and Medical Sensors, (1 September 1991); https://doi.org/10.1117/12.47122

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available