Chirality of the local supercoiling of individual DNA molecules assigned by atomic force microscopy

Bruno Samori; Giuliano Siligardi; Carla Quagliarello; Albrecht L. Weisenhorn; James Vesenka; Carlos J. Bustamante

doi:10.1117/12.146374

4 June 1993 Chirality of the local supercoiling of individual DNA molecules assigned by atomic force microscopy

Bruno Samori, Giuliano Siligardi, Carla Quagliarello, Albrecht L. Weisenhorn, James Vesenka, Carlos J. Bustamante

Proceedings Volume 1855, Scanning Probe Microscopies II; (1993) https://doi.org/10.1117/12.146374
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Reproducible images of pBR322 plasmid molecules have been recorded by Atomic Force Microscopy (AFM) under n-propanol. By applying a stratigraphic analysis which takes advantage of the height information contained in the AFM images, it is possible to assign the chirality of the local supercoiling of the individual molecules.

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Bruno Samori, Giuliano Siligardi, Carla Quagliarello, Albrecht L. Weisenhorn, James Vesenka, and Carlos J. Bustamante "Chirality of the local supercoiling of individual DNA molecules assigned by atomic force microscopy", Proc. SPIE 1855, Scanning Probe Microscopies II, (4 June 1993); https://doi.org/10.1117/12.146374

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