Dynamic light-scattering studies of mucin

Rama Bansil; Sinisa Pajevic; Xingxiang Cao; K. Ramakrishn Bhaskar; Jeffrey Thomas LaMont M.D.; Nezham H. Afdhal; N. Niu

doi:10.1117/12.148358

21 July 1993 Dynamic light-scattering studies of mucin

Rama Bansil, Sinisa Pajevic, Xingxiang Cao, K. Ramakrishn Bhaskar, Jeffrey Thomas LaMont M.D., Nezham H. Afdhal, N. Niu

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148358
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Dynamic light scattering was applied to study aggregation phenomena in mucin, the glycoprotein responsible for the visco-elastic properties of mucus which is found as a lining on most epithelial cell surfaces. Intensity autocorrelation functions measured on purified mucin solutions under varying experimental conditions were analyzed by Laplace inversion methods. The results showed that at low pH (below 4) solutions of gastric mucin contain very large supra-molecular aggregates, with diffusion constants 100 times slower than those of the 2 X 10⁶ molecular weight glycoprotein of mucin. Similar methods were used to investigate the interaction of gall bladder mucin with cholesterol-phospholipid vesicles. Repeated measurements of the intensity correlation functions after adding mucin to a suspension of vesicles showed a two-fold increase in the hydrodynamic radius of the vesicles over a period of three hours after which the vesicle size stayed constant. Control experiments with latex particles in mucin and vesicles in other proteins showed no change in size, implying that the fusion of vesicles is due to vesicle-mucin interactions.

Citation Download Citation

Rama Bansil, Sinisa Pajevic, Xingxiang Cao, K. Ramakrishn Bhaskar, Jeffrey Thomas LaMont M.D., Nezham H. Afdhal, and N. Niu "Dynamic light-scattering studies of mucin", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148358

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