Ultraviolet-laser-induced fluorescence of aerosolized bacterial spores

Steven D. Christesen; Anna Wong; Michael Scott DeSha; Clifton N. Merrow; Mark W. Wilson; John Charles Butler

doi:10.1117/12.144702

18 May 1993 Ultraviolet-laser-induced fluorescence of aerosolized bacterial spores

Steven D. Christesen, Anna Wong, Michael Scott DeSha, Clifton N. Merrow, Mark W. Wilson, John Charles Butler

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144702
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Until quite recently the ability to detect and discriminate aerosolized micro-organisms at long range using the laser induced fluorescence (LIF) technique has met with limited success. The lasers which met our logistic requirements had insufficient energies to propagate through the troposphere and excite a target organism. The detectors, though sensitive enough, did not allow us to see a spectral distribution of the fluorescence return. Advances in laser and detector technology has now brought us higher energy, solid state lasers, and very sensitive array detectors. Using this new technology we built and tested an ultraviolet LIDAR against various interferents and a micro-organic contaminant. In this paper we describe the system and method used to detect and discriminate an aerosolized micro-organism at ranges up to 3 kilometers, and the results of this effort.

Citation Download Citation

Steven D. Christesen, Anna Wong, Michael Scott DeSha, Clifton N. Merrow, Mark W. Wilson, and John Charles Butler "Ultraviolet-laser-induced fluorescence of aerosolized bacterial spores", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144702

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