Paper
18 May 1993 Universal phosphorescence immunoassay
Ekaterina Yu. Mantrova, Marina V. Demcheva, Alexander P. Savitsky, Gely V. Ponomarev
Author Affiliations +
Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144715
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States
Abstract
Pd-coproporphyrin I (Pd-CP) has optimum phosphorescence characteristics for application in immunoassay. The aim of this study is to work out a universal phosphorescence immunoassay method (UniPhIA) using monoclonal antibodies to Pd-CP and conjugates of various proteins with Pd-CP for detection of insulin. Pd-CP and monoclonal antibodies obtained allow a convenient method for determination of various antigens to be developed, which combines the universal character of the amplified enzyme-linked immunosorbent assay (a-ELISA) with a high sensitivity and the simplicity of the time-resolved phosphorescence immunoassay.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ekaterina Yu. Mantrova, Marina V. Demcheva, Alexander P. Savitsky, and Gely V. Ponomarev "Universal phosphorescence immunoassay", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144715
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Phosphorescence

Proteins

Luminescence

Monoclonal antibodies

Lithium

Palladium

Molecules

Back to Top