Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project

Peter W. Williams; David Schieltz; Randall W. Nelson; Chau-Wen Chou; Cong-Wen Luo; Robert Thomas

doi:10.1117/12.146708

24 June 1993 Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project

Peter W. Williams, David Schieltz, Randall W. Nelson, Chau-Wen Chou, Cong-Wen Luo, Robert Thomas

Author Affiliations +

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146708
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Techniques have been developed to volatilize intact massive DNA molecules using pulsed laser ablation of thin frozen films of aqueous DNA solutions. Electrophoresis assay of the ablated DNA shows that molecules as massive as approximately 400,000 Da can be ablated intact. It has been possible to obtain time-of-flight mass spectra of ablated multicomponent mixtures of single-stranded DNA with masses up to approximately 18,000 Da (a 60-nucleotide DNA oligomer). The possible application of time-of-flight mass spectrometry to the analysis and readout of DNA sequence mixtures, and the potential thereby to accelerate the Human Genome project, are discussed.

Citation Download Citation

Peter W. Williams, David Schieltz, Randall W. Nelson, Chau-Wen Chou, Cong-Wen Luo, and Robert Thomas "Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146708

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