Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence

Peter M. Goodwin; Charles W. Wilkerson Jr.; W. Patrick Ambrose; Richard A. Keller

doi:10.1117/12.146731

24 June 1993 Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence

Peter M. Goodwin, Charles W. Wilkerson Jr., W. Patrick Ambrose, Richard A. Keller

Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993) https://doi.org/10.1117/12.146731
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

We report here on the detection and fluorescence lifetime measurement of single Rhodamine- 110 molecules in a flowing, aqueous sample stream. Time-correlated photon counting (TCPC) used in combination with pulsed excitation allows for the detection, in the presence of significant prompt Raman and Rayleigh background, of photon bursts due to single fluorescent molecules passing through a small detection volume (approximately 1 pL). The fluorescence lifetime of a detected molecule is estimated from the decay curve complied from photon arrival times in the burst.

Citation Download Citation

Peter M. Goodwin, Charles W. Wilkerson Jr., W. Patrick Ambrose, and Richard A. Keller "Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); https://doi.org/10.1117/12.146731

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