Time-resolved transient absorption and photoresponse studies of oriented bacteriorhodopsin in Langmuir-Blodgett films

Helge Lemmetyinen; Marjo Ikonen; Alexey Sharonov; Nikolai V. Tkachenko

doi:10.1117/12.146121

17 June 1993 Time-resolved transient absorption and photoresponse studies of oriented bacteriorhodopsin in Langmuir-Blodgett films

Helge Lemmetyinen, Marjo Ikonen, Alexey Sharonov, Nikolai V. Tkachenko

Author Affiliations +

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146121
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

In this work we have studied photon initiated processes of bacteriorhodopsin (BR) in Langmuir-Blodget (LB) monolayers and multilayers. As far as we know the present work is the first, where the photoresponse function and kinetics of BR is detected from a monolayer as well as the first report, where oriented monolayers have been used as a proton pump. The transient absorption spectra and the decay functions of multilayers were detected for films in water, in water vapor saturated air, and at room humidity. This had an influence on transient lifetimes. The method we have used to orient the bacteriorhodopsin molecules is based on the LB-technique and the fact that the protein orients during the coating process when a phospholipid binder is used. The photoelectric signals of the monolayers deposited by the different directions had opposite polarities. The amplitude due to five layers was five times as high as that due to a monolayer.

Citation Download Citation

Helge Lemmetyinen, Marjo Ikonen, Alexey Sharonov, and Nikolai V. Tkachenko "Time-resolved transient absorption and photoresponse studies of oriented bacteriorhodopsin in Langmuir-Blodgett films", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146121

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available