Liposome-administered tetramethylhematoporphyrin (TMHP) as a photodynamic agent

Ella Dumbravia Reich; Ruediger Bachor; Kurt Miller M.D.; Karsten Koenig; Denes Repassy; Richard E. Hautmann

doi:10.1117/12.168657

1 March 1994 Liposome-administered tetramethylhematoporphyrin (TMHP) as a photodynamic agent

Ella Dumbravia Reich, Ruediger Bachor, Kurt Miller M.D., Karsten Koenig, Denes Repassy, Richard E. Hautmann

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168657
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The purpose of these studies was to determine whether liposomes can deliver the photo- sensitizer TMHP to human bladder carcinoma cells and fibroblast cells, and how effective the photodynamic activity of this photosensitizer is. TMHP was incorporated into small unilamellar liposomes of DPPC. Cellular uptake of TMHP was estimated after extraction with 0.1 N NaOH and by using a fluorescence microscope. Quantitative levels of TMHP in the three cell lines have been expressed in terms of (mu) g per 1.10⁶ cells. PDT was performed for one hour after sensitization using an argon-pumped dye laser at 630 nm. Compared to the fibroblasts, neither a selective uptake of TMHP nor an increased effect of phototoxicity did occur in the tumor cell lines. PDT efficiency is dependent on cell line, dose and fluence rate.

Citation Download Citation

Ella Dumbravia Reich, Ruediger Bachor, Kurt Miller M.D., Karsten Koenig, Denes Repassy, and Richard E. Hautmann "Liposome-administered tetramethylhematoporphyrin (TMHP) as a photodynamic agent", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168657

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