Laser diffraction of RBC: the method and its pitfalls

Rainer Bayer; Serdar Caglayan; Rainer Hofmann; Dieter Ostuni

doi:10.1117/12.179022

28 June 1994 Laser diffraction of RBC: the method and its pitfalls

Rainer Bayer, Serdar Caglayan, Rainer Hofmann, Dieter Ostuni

Proceedings Volume 2100, Cell and Biotissue Optics: Applications in Laser Diagnostics and Therapy; (1994) https://doi.org/10.1117/12.179022
Event: Volga Laser Tour '93, 1993, Moscow, Russian Federation

Abstract

The method to determine red blood cell deformability by laser diffraction is presented. It combines ektacytometry (laser diffraction) with image analysis. Laser light is passed through erythrocyte suspensions, which are sheared in a Searle like viscometer. The laser diffraction patterns are photographed by a CCD camera and further analyzed by a computer. Flexibility is characterized by the quotient of minor and major axes of isointensity lines of the elliptically transformed diffraction patterns. The variation coefficient of the measurement is less than 0.5%.

Citation Download Citation

Rainer Bayer, Serdar Caglayan, Rainer Hofmann, and Dieter Ostuni "Laser diffraction of RBC: the method and its pitfalls", Proc. SPIE 2100, Cell and Biotissue Optics: Applications in Laser Diagnostics and Therapy, (28 June 1994); https://doi.org/10.1117/12.179022

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available