Novel reduced nicotinamide adenine dinucleotide optical sensor

Ashutosh Sharma

doi:10.1117/12.180770

28 July 1994 Novel reduced nicotinamide adenine dinucleotide optical sensor

Ashutosh Sharma

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180770
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A novel method for the fluorometric measurement of an analyte is described that is based on the anti-Stokes fluorescence of an indicator. The validity of the concept is demonstrated by developing a sensitive assay for NADH. Thionine, a thiazine dye, when excited using radiation of a wavelength 630 nm, emits a broad fluorescence, peaking at 620 nm. Similar to the Stokes fluorescence, this anti-Stokes' fluorescence of thionine is efficiently quenched by NADH. Stern-Volmer plots of the quenching data reveal a quenching constant of 0.7 X 10³ M^-1. The process of quenching is attributed to a photo-reaction between the excited thionine and NADH, resulting in NAD⁺.

Citation Download Citation

Ashutosh Sharma "Novel reduced nicotinamide adenine dinucleotide optical sensor", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180770

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