Interaction of 7-aminoactinomycin D with single- and double-stranded DNA oligonucleotides

Susan A. Bailey; David E. Graves; Maurice R. Eftink

doi:10.1117/12.182756

17 August 1994 Interaction of 7-aminoactinomycin D with single- and double-stranded DNA oligonucleotides

Susan A. Bailey, David E. Graves, Maurice R. Eftink

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182756
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

7-Aminoactinomycin D is a potent inhibitor of RNA polymerase and it has been demonstrated to bind to a single stranded DNA. Wadkins and Jovin showed that there is a 20 to 40 fold enhancement in the fluorescence of 7-aminoactinomycin D upon binding to certain single stranded DNA sequences. We report studies of the thermodynamics of the binding of the parent drug, actinomycin D, to single and double stranded DNA. A five-fold range of association constants is found for the different binding sequences that we have investigated. We will correlate these thermodynamic studies with measurements of the fluorescence enhancement, lifetime, and rotational correlation time of the drug bound to the various single and double stranded oligonucleotides.

Citation Download Citation

Susan A. Bailey, David E. Graves, and Maurice R. Eftink "Interaction of 7-aminoactinomycin D with single- and double-stranded DNA oligonucleotides", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182756

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