Fluorescence excitation and emission spectra of ALA-induced protoporphyrin IX in normal and tumoral tissue of the human bladder

Martin Forrer; Thomas M. Glanzmann; Jerome C. Mizeret; Daniel Braichotte; Georges A. Wagnieres; Hubert van den Bergh; Patrice Jichlinski; Hans-Juerg Leisinger M.D.

doi:10.1117/12.198705

4 January 1995 Fluorescence excitation and emission spectra of ALA-induced protoporphyrin IX in normal and tumoral tissue of the human bladder

Martin Forrer, Thomas M. Glanzmann, Jerome C. Mizeret, Daniel Braichotte, Georges A. Wagnieres, Hubert van den Bergh, Patrice Jichlinski, Hans-Juerg Leisinger M.D.

Author Affiliations +

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198705
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

In vivo spectrofluorometric analysis represents a tool to obtain information about fluorophore distribution in tissue. Based on a Peltier-cooled CCD we designed a fluorescence excitation and emission spectrograph which allows to obtain tissue spectra endoscopically and in a clinical environment. Clinical studies were performed on patients with positive cytology or tumor recurrence in the urinary bladder. Patients received a 50 ml instillation of 3% ALA solution at pH 5.5 during 3 to 4 hours and underwent a normal white light cystoscopic examination together with light induced fluorescence photodetection at 5 to 8 hours after the beginning of the instillation. Local fluorescence measurements with a single fiber were performed before photodetection. These showed fluorescence ratios between tumor and normal tissue of 1.5 to 20 with the strongest ratios for exophytic papillary tumors. Fluorescence excitation between 380 nm and 450 nm revealed that the higher Protoporphyrin IX (PPIX) signal on tumor tissue is accompanied by a decrease of the autofluorescence at the emission wavelength of 500 nm.

Citation Download Citation

Martin Forrer, Thomas M. Glanzmann, Jerome C. Mizeret, Daniel Braichotte, Georges A. Wagnieres, Hubert van den Bergh, Patrice Jichlinski, and Hans-Juerg Leisinger M.D. "Fluorescence excitation and emission spectra of ALA-induced protoporphyrin IX in normal and tumoral tissue of the human bladder", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198705

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