Fluorescence detection of premalignant, malignant, and micrometastatic disease using hexylpyropheophorbide

Kinya Furukawa M.D.; David H. Crean; Thomas S. Mang; Harubumi Kato M.D.; Thomas J. Dougherty

doi:10.1117/12.203403

1 March 1995 Fluorescence detection of premalignant, malignant, and micrometastatic disease using hexylpyropheophorbide

Kinya Furukawa M.D., David H. Crean, Thomas S. Mang, Harubumi Kato M.D., Thomas J. Dougherty

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203403
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

We investigated the in vivo fluorescence detection of premalignant and malignant lesions in carcinogen-induced tumors of the hamster buccal cheek pouch and micrometastases in rat lymph nodes using hexylpyropheophorbide-a (HPPH) at 0.5 mg/kg and He-Ne laser-induced fluorescence photometry. Time course studies of HPPH in hamster tumor model showed maximal relative fluorescence readings at 48 hours after i.p. injection in each stage (dysplasia: 0.35, papilloma: 0.58, squamous cell carcinoma: 1.04). Squamous cell carcinoma showed significantly greater fluorescence than papillomas and dysplasias at all time points (p < 0.01). Metastatic lymph nodes were significantly greater than normal lymph nodes at all time points (p < 0.01). Maximal fluorescence levels of normal lymph nodes were observed at 6 hours after i.v. injection. Metastatic lymph nodes still showed high fluorescence levels at 72 hours. Micrometastases showed fluorescence levels between the levels of metastatic and normal lymph nodes (normal: 0.40 < micrometastasis: 0.62 < metastasis: 0.75 at 48 hours after i.v. injection). The results demonstrated good correlation between fluorescence levels and histopathological developments at all time points. Therefore, HPPH may be a promising fluorophore for the detection of developing malignancies and occult disease.

Citation Download Citation

Kinya Furukawa M.D., David H. Crean, Thomas S. Mang, Harubumi Kato M.D., and Thomas J. Dougherty "Fluorescence detection of premalignant, malignant, and micrometastatic disease using hexylpyropheophorbide", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203403

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