Intracavity frequency doubling of a continuous wave Ti:sapphire ring laser and application in resonance Raman spectroscopy of heme protein dynamics

Scott C. Buchter; Curtis Williams; Alfons Schulte; Theodore Alekel III; Gregory J. Mizell; William R. Fay

doi:10.1117/12.206943

17 April 1995 Intracavity frequency doubling of a continuous wave Ti:sapphire ring laser and application in resonance Raman spectroscopy of heme protein dynamics

Scott C. Buchter, Curtis Williams, Alfons Schulte, Theodore Alekel III, Gregory J. Mizell, William R. Fay

Author Affiliations +

Proceedings Volume 2380, UV and Visible Lasers and Laser Crystal Growth; (1995) https://doi.org/10.1117/12.206943
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Noncritical temperature-tuned phase-matching and large nonlinear coefficients make potassium niobate an attractive material for frequency doubling tuneable near-infrared radiation. We have mounted a KNbO₃ crystal intracavity in an argon ion pumped, continuous wave Ti:Sapphire ring laser to increase the power level of the second harmonic. Wavelength selection at the fundamental frequency is accomplished with a birefringent filter. By using the crystal orientation that defines the d₃₂ coefficient of KNbO₃ we have obtained a blue second harmonic output tuneable from 425-445 nm. The laser is also characterized by the narrow linewidth of the Ti:Sapphire ring oscillator and good temporal stability. A continuous wave, frequency doubled Ti:sapphire laser is well suited to excite the resonance Raman spectrum in heme proteins with strong absorption bands in the range of 400 to 450 nm. We demonstrate the feasibility of such a setup for Raman studies of ligand binding to myoglobin. The Raman bands yield information on the reaction dynamics and on conformational changes near the linkage between the heme and the protein. In particular, a shift of the stretch frequency of the iron- histidine bond with high pressure may be attributed to a protein conformational change.

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Scott C. Buchter, Curtis Williams, Alfons Schulte, Theodore Alekel III, Gregory J. Mizell, and William R. Fay "Intracavity frequency doubling of a continuous wave Ti:sapphire ring laser and application in resonance Raman spectroscopy of heme protein dynamics", Proc. SPIE 2380, UV and Visible Lasers and Laser Crystal Growth, (17 April 1995); https://doi.org/10.1117/12.206943

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KEYWORDS

Raman spectroscopy

Proteins

Crystals

Continuous wave operation

Sapphire lasers

Optical filters

Second-harmonic generation

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