Effect of wavelength and dye selection on biosensor response

Frances S. Ligler; Kristen A. Breslin; Lynn Kim Cao; George P. Anderson

doi:10.1117/12.208480

8 May 1995 Effect of wavelength and dye selection on biosensor response

Frances S. Ligler, Kristen A. Breslin, Lynn Kim Cao, George P. Anderson

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208480
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

The availability of low cost laser diodes and new fluorescent dyes has made portable biosensors a reality. Previously, we have examined the variation in the fluorescent signal generated in an antigen-antibody reaction when the antigen is labeled with dyes exciting at different wavelengths. In this study, we looked at the effect of changing dyes and wavelengths on a sandwich immunoassay for the F1 antigen from Yersinia pestis, the etiologic agent of plaque. The F1 immunoassay has previously been demonstrated to work in serum, plasma, and even whole blood, when performed using a fiber optic biosensor. In this study, we demonstrated that changing to cyanine dyes enhanced the sensitivity of the detection without altering the immunochemistry of the assay.

Citation Download Citation

Frances S. Ligler, Kristen A. Breslin, Lynn Kim Cao, and George P. Anderson "Effect of wavelength and dye selection on biosensor response", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208480

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