High throughput screening using dynamic fluorescence

Kerry M. Swift; Edmund D. Matayoshi

doi:10.1117/12.208476

8 May 1995 High throughput screening using dynamic fluorescence

Kerry M. Swift, Edmund D. Matayoshi

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208476
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Multifrequency phase-modulation data for fluorescence lifetime and anisotropy decay standards have been obtained for liquid samples in 96-well microtiter plates. We coupled the bifurcated fiber optic probe of a fluorescence plate reader to a phase and modulation time- resolving fluorometer. Laser excitation was used and multifrequency data could be acquired quickly over a range of modulation frequencies from 15 to greater than 150 MHz, at each well position, through the use of multiharmonic Fourier methods. We evaluated the system's performance for UV-excited fluorescence, with the goal of screening quality or properties of proteins or peptides by steady-state or dynamic intrinsic fluorescence intensity or anisotropy using the high throughput 96-well plate format.

Citation Download Citation

Kerry M. Swift and Edmund D. Matayoshi "High throughput screening using dynamic fluorescence", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208476

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