Detection of mercury compounds using invertase-glucose oxidase-based biosensor

A. Amine; C. Cremisini; Giuseppe Palleschi

doi:10.1117/12.224103

9 October 1995 Detection of mercury compounds using invertase-glucose oxidase-based biosensor

A. Amine, C. Cremisini, Giuseppe Palleschi

Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224103
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

Mercury compounds have been determined with an electrochemical biosensor based on invertase inhibition. When invertase is in the presence of mercury its activity decreases; this causes a decrease of glucose production which is monitored by the glucose sensor and correlated to the concentration of mercury in solution. Parameters as pH, enzyme concentration, substrate concentration, and reaction and incubation time were optimized. Mercury compounds determination using soluble or immobilized invertase were reported. Results show that the inhibition was competitive and reversible. Mercury compounds can be detected directly in aqueous solution in the range 2 - 10 ppb.

Citation Download Citation

A. Amine, C. Cremisini, and Giuseppe Palleschi "Detection of mercury compounds using invertase-glucose oxidase-based biosensor", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224103

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