Cultured human cornea healing process after free-electron-laser ablation

Jin-Hui Shen; Karen Margaret Joos M.D.; Debra J. Shetlar; Richard D. Robinson; Gurpreet K. Thind; Glenn S. Edwards; Denis M. O'Day M.D.

doi:10.1117/12.275106

26 May 1997 Cultured human cornea healing process after free-electron-laser ablation

Jin-Hui Shen, Karen Margaret Joos M.D., Debra J. Shetlar, Richard D. Robinson, Gurpreet K. Thind, Glenn S. Edwards, Denis M. O'Day M.D.

Author Affiliations +

Proceedings Volume 2971, Ophthalmic Technologies VII; (1997) https://doi.org/10.1117/12.275106
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The purpose of this study is to investigate the healing process in cultured human cornea after infrared Free Electron Laser ablation. Fresh human cadaver cornea was ablated using the Free Electron Laser at the amide II band peak (6.45 micrometers ). The cornea was then cultured in an incubator for 18 days. Haze development within the ablated area was monitored during culture. Histologic sections of the cornea showed complete re-epithelialization of the lased area, and ablation of the underlying Bowman's layer and stroma. The endothelium appeared unaffected. Cultured human corneas may provide useful information regarding the healing process following laser ablation.

Citation Download Citation

Jin-Hui Shen, Karen Margaret Joos M.D., Debra J. Shetlar, Richard D. Robinson, Gurpreet K. Thind, Glenn S. Edwards, and Denis M. O'Day M.D. "Cultured human cornea healing process after free-electron-laser ablation", Proc. SPIE 2971, Ophthalmic Technologies VII, (26 May 1997); https://doi.org/10.1117/12.275106

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