Fiber optic light-scattering measurement system for evaluation of embryo viability: light-scattering characteristics from live mouse embryo

Harumi Itoh; Tsunenori Arai; Makoto Kikuchi

doi:10.1117/12.275464

16 June 1997 Fiber optic light-scattering measurement system for evaluation of embryo viability: light-scattering characteristics from live mouse embryo

Harumi Itoh, Tsunenori Arai, Makoto Kikuchi

Author Affiliations +

Proceedings Volume 2975, Laser-Tissue Interaction VIII; (1997) https://doi.org/10.1117/12.275464
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We measured angular distribution of the light scattering from live mouse embryo with 632.8nm in wavelength to evaluate the embryo viability. We aim to measure the mitochondrial density in human embryo which have relation to the embryo viability. We have constructed the light scattering measurement system to detect the mitochondrial density non-invasively. We have employed two optical fibers for the illumination and sensing to change the angle between these fibers. There were two dips on the scattering angular distribution from the embryo. These dips existed on 30 and 85 deg. We calculated the scattering angular pattern by Mie theory to fit the measured scattering estimated scattering size and density. The best fitting was obtained when the particle size and density were 0.9 micrometers and 10¹⁰ particles per ml, respectively. These values coincided with the approximated values of mitochondrial in the embryo. The measured light scattering may mainly originated from mitochondria in spite of the existence of the various scattering particles in the embryo. Since our simple scattering measurement may offer the mitochondrial density in the embryo, it might become the practical method of human embryo on in vitro fertilization-embryo transfer.

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Harumi Itoh, Tsunenori Arai, and Makoto Kikuchi "Fiber optic light-scattering measurement system for evaluation of embryo viability: light-scattering characteristics from live mouse embryo", Proc. SPIE 2975, Laser-Tissue Interaction VIII, (16 June 1997); https://doi.org/10.1117/12.275464

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