S-layers as patterning structures and supporting layers for biomimetic membranes

Dietmar Pum; Barbara Wetzer; Bernhard Schuster; Uwe B. Sleytr

doi:10.1117/12.269979

31 March 1997 S-layers as patterning structures and supporting layers for biomimetic membranes

Dietmar Pum, Barbara Wetzer, Bernhard Schuster, Uwe B. Sleytr

Proceedings Volume 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications; (1997) https://doi.org/10.1117/12.269979
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A new approach in nanostructure technology particularly in the functionalization of surfaces has been developed on the basis of crystalline bacterial cell surface layers (S- layers). S-layers are composed of monomolecular arrays of identical (glyco)proteins showing high molecular order, defined mass distribution and isoporosity, and a high binding capacity for functional macromolecules. The possibility for recrystallizing isolated S-layer subunits into large isoporous, coherent lattices at solid supports, at the air/water interface or on lipid films and for handling such layers by standard Langmuir-Blodgett techniques opens a broad spectrum of applications in basic and applied membrane research. S-layer supported functional phospholipid bilayers or tetraether lipid films mimic the molecular architecture of those archaebacterial cell envelopes that are exclusively composed of an S-layer and a plasma membrane. This novel concept could lead to new techniques for exploiting large scale structural and functional principles of membrane associated and integrated molecules (e.g. ion channels, proton pumps, receptors).

Citation Download Citation

Dietmar Pum, Barbara Wetzer, Bernhard Schuster, and Uwe B. Sleytr "S-layers as patterning structures and supporting layers for biomimetic membranes", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); https://doi.org/10.1117/12.269979

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