Fluorescence-lifetime imaging using a novel photon sensing module

David McLoskey; Klaus Suhling; David J. S. Birch

doi:10.1117/12.273544

7 May 1997 Fluorescence-lifetime imaging using a novel photon sensing module

David McLoskey, Klaus Suhling, David J. S. Birch

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273544
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We report the first read-out module for use with single- photon timing array detectors such as multi-anode MCP-PMTs. The IBH Model 5000MXR interfaces to the time-correlated single-photon counting (TCSPC) technique using a single time-to-amplitude converter. In addition to performing established multiplexing tasks, such as simultaneous acquisition of fluorescence and excitation and anisotropy, the new module enables spectral and spatial imaging of kinetic parameters such as fluorescence lifetimes and amplitudes. The system retains the inherent advantages of TCSPC with respect to picosecond time resolution and wide dynamic range, while featuring parallel data acquisition and enhanced data acquisition rates. Unlike early TTL implementations of multiplexing which were limited to four channels, our system uses an application specific integrated circuit (ASIC) which can read out the data from up to sixteen detection channels with higher reliability and less time-dispersion. The Model 5000MXR can be packaged as a NIM standard module, packaged to serve more channels or be close coupled to detector arrays for specific applications such as microscopy and lifetime based sensors. The theory, design and performance of ASIC data read-out will be described. Other applications include photon migration in tissue, time- of-flight reflectometry/mass spectrometry and nucleonics.

Citation Download Citation

David McLoskey, Klaus Suhling, and David J. S. Birch "Fluorescence-lifetime imaging using a novel photon sensing module", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273544

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