X-ray imaging of living cells

Toshikazu Majima; Hideaki Shimizu; Toshihisa Tomie; Eisuke Miura; Toshihiko Kanayama; Masahiro Yamada

doi:10.1117/12.274326

23 May 1997 X-ray imaging of living cells

Toshikazu Majima, Hideaki Shimizu, Toshihisa Tomie, Eisuke Miura, Toshihiko Kanayama, Masahiro Yamada

Proceedings Volume 2983, Functional Imaging and Optical Manipulation of Living Cells; (1997) https://doi.org/10.1117/12.274326
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

X-ray images of living cells were obtained by using a flash contact x-ray microscope system. The system consists of a high power laser (grass laser or KrF laser) for producing a plasma as an x-ray source, a small vacuum chamber, and a sample holder with a metal target for making plasma. The x- ray images were recorded on an x-ray sensitive layer on a silicon wafer. After chemical development x-ray images on the x-ray sensitive layers were read out by using an atomic force microscope. The performance of the system is demonstrated by presenting x-ray images of unicellular microorganisms, bacteria and skeletal muscle fibers. The intracellular structures were visible in some cases. For laboratory use a flash contact table-top x-ray microscopy is improved using a table-scaled YAG laser. The table-top systems showed good performance comparing to that of the x- ray microscope system with high power laser. These results indicate that the table-top x-ray microscope is a powerful instrumentation for the observation of living cells.

Citation Download Citation

Toshikazu Majima, Hideaki Shimizu, Toshihisa Tomie, Eisuke Miura, Toshihiko Kanayama, and Masahiro Yamada "X-ray imaging of living cells", Proc. SPIE 2983, Functional Imaging and Optical Manipulation of Living Cells, (23 May 1997); https://doi.org/10.1117/12.274326

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available