Reabsorption effects on calibration of fluorescence-lifetime-based sensors

Andrea Kobe; Ales Babnik; Janez Ivan Mozina

doi:10.1117/12.281249

24 September 1997 Reabsorption effects on calibration of fluorescence-lifetime-based sensors

Andrea Kobe, Ales Babnik, Janez Ivan Mozina

Proceedings Volume 3099, Micro-optical Technologies for Measurement, Sensors, and Microsystems II and Optical Fiber Sensor Technologies and Applications; (1997) https://doi.org/10.1117/12.281249
Event: Lasers and Optics in Manufacturing III, 1997, Munich, Germany

Abstract

Intrinsic advantages of fluorescence lifetime based optical sensors, as referred in the literature, are their stability towards source fluctuations and self-reference. The latter can be interpreted also as non-dependence on geometrical design and other properties of the constructed sensor. In the article presented it is shown this is not the case. The measured fluorescence decay time, which should be unambiguously connected to the measured quantity (temperature, for example), shows an intricate dependence on source, geometrical properties and signal processing technique. Numerical calculations together with theoretical discussion are employed to quantize and interpret this effects, mainly caused by reabsorption. Different sensor designs are compared, an optimal sensor construction suggested and alternative digital signal processing technique introduced.

Citation Download Citation

Andrea Kobe, Ales Babnik, and Janez Ivan Mozina "Reabsorption effects on calibration of fluorescence-lifetime-based sensors", Proc. SPIE 3099, Micro-optical Technologies for Measurement, Sensors, and Microsystems II and Optical Fiber Sensor Technologies and Applications, (24 September 1997); https://doi.org/10.1117/12.281249

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