Bacteriorhodopsin-based single- and dual-wavelength interferometry for monitoring crystal growth

Colleen Mary Fitzpatrick; Ching Mei Yang; Dominique C. Fourguette

doi:10.1117/12.277709

7 July 1997 Bacteriorhodopsin-based single- and dual-wavelength interferometry for monitoring crystal growth

Colleen Mary Fitzpatrick, Ching Mei Yang, Dominique C. Fourguette

Proceedings Volume 3123, Materials Research in Low Gravity; (1997) https://doi.org/10.1117/12.277709
Event: Optical Science, Engineering and Instrumentation '97, 1997, San Diego, CA, United States

Abstract

Bacteriorhodopsin-based interferometry is a new technique with which high resolution interferograms can be recorded at one or more wavelengths in real time. The erasable nature of bacteriorhodopsin, its panchromatic response to light, and the fact that recording is done on the molecular level, allow for the formation of fringe patterns combining the high resolution of conventional silver halide recording materials with the real time properties of CCD cameras. In this context, we have used bacteriorhodopsin to image changes in the crystal environment at single and dual wavelengths, using a real time sequencing architecture whereby successive exposures of a bR thin film are overlapped to produce a continuous stream of interferometric images. This allows real-time data buffering and immediate, on-line observation of results. In this paper, we present real time single and dual wavelength interferograms of growing KAl(SO₄)₂ and melting sugar crystals recorded on bacteriorhodopsin thin films. The interferograms were produced with a shuttered cw argon or a pulsed Nd:YAG write laser, and a helium neon read laser, and show enhanced contrast and resolution. The results of this effort clearly demonstrate the superior, real time recording capabilities of bacteriorhodopsin thin films.

Citation Download Citation

Colleen Mary Fitzpatrick, Ching Mei Yang, and Dominique C. Fourguette "Bacteriorhodopsin-based single- and dual-wavelength interferometry for monitoring crystal growth", Proc. SPIE 3123, Materials Research in Low Gravity, (7 July 1997); https://doi.org/10.1117/12.277709

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