Alteration of mitochondrial membrane potential in photosensitized cardiocytes monitored by fast-response fluorescent probe

Mariusz Adamek; Asher Shainberg; Fidi Schwartz; Shimon Rochkind

doi:10.1117/12.297796

29 December 1997 Alteration of mitochondrial membrane potential in photosensitized cardiocytes monitored by fast-response fluorescent probe

Mariusz Adamek, Asher Shainberg, Fidi Schwartz, Shimon Rochkind

Author Affiliations +

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297796
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Mitochondria constitute well-established target for photodynamic action provided that duration of incubation time with hydrophobic photosensitizer is long enough (18 - 24 hrs) to enable incorporation of the dye into these organelles. It was found that 1 hour incubation of rat cardiocytes with hematoporphyrin dihydrochloride (Hp) followed by low-energy HeNe laser irradiation (ED: 0.3, 1.0, or 3.0 J/cm²), affected mitochondrial membrane potential. The latter was monitored by means of the fast-response fluorescent probe DASPMI and the dynamic video imaging system equipped with frame-grabbing and analyzing software. The time-course of mitochondrial membrane potential decrease exhibited dependence on both: Hp concentration and ED used. In cells incubated with 1 (mu) g/ml Hp a decrease of DASPMI fluorescence occurred during 8 - 15 mins and was preceded by oscillations of fluorescence intensity. For cells incubated with 100 (mu) g/ml Hp disappearance of discrete pattern of mitochondrial fluorescence was observed 1 - 2 mins after irradiation.

Citation Download Citation

Mariusz Adamek, Asher Shainberg, Fidi Schwartz, and Shimon Rochkind "Alteration of mitochondrial membrane potential in photosensitized cardiocytes monitored by fast-response fluorescent probe", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297796

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