In-vitro studies of zinc(II)-phthalocyanine (Zn-PC) for use in photochemotherapy

G. H. Rodal; Johan Moan; Kristian Berg

doi:10.1117/12.297816

29 December 1997 In-vitro studies of zinc(II)-phthalocyanine (Zn-PC) for use in photochemotherapy

G. H. Rodal, Johan Moan, Kristian Berg

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297816
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In the present study cellular uptake and intracellular localization of a liposomal formulation of Zn-PC, was studied in human cervix carcinoma cells of the line NHIK 3025. The cellular uptake of Zn-PC was found to be completed after 4 hours of incubation. Seventy to eighty percent of the dye taken up by the cells during 18 hours of incubation was retained after 1 hour of further incubation in sensitizer-free medium. The cellular uptake was attenuated by the presence of serum and at low temperature of the incubation medium. The uptake was not enhanced by decreasing the pH of the incubation medium. The present study indicated that Zn-PC is rapidly transferred from liposomes to lipoproteins. Analysis of inactivation of subcellular marker enzymes indicated that Zn- PC is located in the Golgi apparatus and mitochondria.

Citation Download Citation

G. H. Rodal, Johan Moan, and Kristian Berg "In-vitro studies of zinc(II)-phthalocyanine (Zn-PC) for use in photochemotherapy", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297816

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