Modular diffuse reflection and fluorescence emission imaging colorimeter for the in-vivo study of parameters related to the phototoxic effect in PDT

Constantin J. Balas; Maria P. Stefanidou; Tarsi C. Giannouli; Savas K. Georgiou; Emmanuel S. Helidonis M.D.; Andronicki D. Tosca

doi:10.1117/12.297830

29 December 1997 Modular diffuse reflection and fluorescence emission imaging colorimeter for the in-vivo study of parameters related to the phototoxic effect in PDT

Constantin J. Balas, Maria P. Stefanidou, Tarsi C. Giannouli, Savas K. Georgiou, Emmanuel S. Helidonis M.D., Andronicki D. Tosca

Author Affiliations +

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297830
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

The biological response to PDT depends on the photosensitizer accumulation to the malignant cells, the tumor vasculature, the inherent cell sensitivity to the photodynamic effect, etc. The in-vivo measurement of physical parameters related with these factors can serve in the understanding of the mechanisms involved in the cell death, as well as in the optimization of the treatment procedure. In this paper we demonstrate a modular diffuse reflection and fluorescence emission imaging colorimeter. We have used it for the in-vivo on-line quantitative measurement and mapping the time course fluorescence intensity after ALA application and for the tissue color changes associated with the erythema developed during irradiation. Based on the obtained quantitative data, we investigate the correlation between the photosensitizer accumulation before light irradiation and the hemodynamic changes, revealed as erythema development, occurring during light irradiation. Their relative significance as predicting factors for the photodynamic treatment effectiveness is also evaluated.

Citation Download Citation

Constantin J. Balas, Maria P. Stefanidou, Tarsi C. Giannouli, Savas K. Georgiou, Emmanuel S. Helidonis M.D., and Andronicki D. Tosca "Modular diffuse reflection and fluorescence emission imaging colorimeter for the in-vivo study of parameters related to the phototoxic effect in PDT", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297830

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