New developments in fluorescence detection of ALA-induced protoporphyrin IX for cancer localization

Herbert G. Stepp; Reinhold Baumgartner; Christian Betz; Karl Bise; P. Brand; Fernando Gamarra; Karl Haeussinger; Peter Hillemanns; Rudolf M. Huber; Ruth Knuechel; M. Kriegmair; Andreas Leunig; J. Pichler; Kai Rick; H. Schulz; F. Stanzel; Susanne Stocker; Simon Wagner; H. Weigandt

doi:10.1117/12.297952

29 December 1997 New developments in fluorescence detection of ALA-induced protoporphyrin IX for cancer localization

Herbert G. Stepp, Reinhold Baumgartner, Christian Betz, Karl Bise, P. Brand, Fernando Gamarra, Karl Haeussinger, Peter Hillemanns, Rudolf M. Huber, Ruth Knuechel, M. Kriegmair, Andreas Leunig, J. Pichler, Kai Rick, H. Schulz, F. Stanzel, Susanne Stocker, Simon Wagner, H. Weigandt

Author Affiliations +

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297952
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

After the very promising clinical results for the detection of bladder cancer in urology, preclinical and clinical studies on aminolevulinic acid (5-ALA) induced protoporphyrin IX (PPIX) are preformed in various disciplines now. This paper provides a brief overview of the progress on 5-ALA assisted fluorescence diagnosis in urology, pulmonology, neurosurgery, gynecology and ENT performed in collaboration with the Laser Research Laboratory at the Department of Urology of the Ludwig-Maximilians-University in Munich. Five-ALA can be applied either topically or systemically to induce an intracellular accumulation of fluorescing PPIX. With appropriate dosage of 5-ALA, malignant tissue can be stained selectively, and irradiation with violet light excites a bright red fluorescence of the tumor. Optical properties of the tissue tend to hamper the precise identification and demarcation of suspect areas in fluorescence images. Multicolor remission and fluorescence imaging, therefore, seems to be indispensable for a reliable tumor localization.

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Herbert G. Stepp, Reinhold Baumgartner, Christian Betz, Karl Bise, P. Brand, Fernando Gamarra, Karl Haeussinger, Peter Hillemanns, Rudolf M. Huber, Ruth Knuechel, M. Kriegmair, Andreas Leunig, J. Pichler, Kai Rick, H. Schulz, F. Stanzel, Susanne Stocker, Simon Wagner, and H. Weigandt "New developments in fluorescence detection of ALA-induced protoporphyrin IX for cancer localization", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297952

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