Laser tweezers as novel nonlinear tools in cell and biomolecular diagnostics

Karsten Koenig

doi:10.1117/12.301127

1 January 1998 Laser tweezers as novel nonlinear tools in cell and biomolecular diagnostics

Karsten Koenig

Proceedings Volume 3199, Biomedical Systems and Technologies II; (1998) https://doi.org/10.1117/12.301127
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In cell and molecular biology, single-beam gradient force optical traps (`laser tweezers') are mainly used as 3D micromanipulation tools, e.g. in controlled cell transport. Otherwise, near infrared (NIR) laser tweezers may act as useful diagnostic tools. They can be used (1) for sensitive measurements on highly-motile cells or organelles by sample confinement within the focal volume, (2) as force transducer, and (3) as linear and nonlinear excitation source. In the case of cells with one-photon NIR absorbers, such as erythrocytes, linear NIR excitation of hemoglobin can result in photothermal hemolysis. In addition, two- photon excitation of a variety of endogenous cellular absorbers, such as NAD(P)H, flavin and porphyrins, occurs de to the high photon flux densities in optical traps. Two- photon NIR excited visible fluorescence of endogenous as well as within a sub-femtoliter focal volume allows a novel nonlinear fluorescence diagnostics of cells and biomolecules.

Citation Download Citation

Karsten Koenig "Laser tweezers as novel nonlinear tools in cell and biomolecular diagnostics", Proc. SPIE 3199, Biomedical Systems and Technologies II, (1 January 1998); https://doi.org/10.1117/12.301127

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