Effect of laser irradiation on the functional activity of enzymes with different structural complexity

Svetlana A. Ostrovtsova; Alexander P. Volodenkov; Alexander A. Maskevich; Irina M. Artsukevich; Slavomir S. Anufrik; Alexander F. Makarchikov; Ivan P. Chernikevich; Vitali I. Stepuro

doi:10.1117/12.308213

18 May 1998 Effect of laser irradiation on the functional activity of enzymes with different structural complexity

Svetlana A. Ostrovtsova, Alexander P. Volodenkov, Alexander A. Maskevich, Irina M. Artsukevich, Slavomir S. Anufrik, Alexander F. Makarchikov, Ivan P. Chernikevich, Vitali I. Stepuro

Author Affiliations +

Proceedings Volume 3255, Applications of Ultrashort-Pulse Lasers in Medicine and Biology; (1998) https://doi.org/10.1117/12.308213
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Three enzymes differing in their structural composition were irradiated by UV lasers to study the effect of temperature, protein concentration and addition of small molecules on their sensitivity to radiation exposure. The laser-induced effects were due to the structural complexity of the protein molecules and depended on the dose applied, the wavelength and the density of irradiation. The multi-enzyme 2- oxoglutarate dehydrogenase complex was subjected to pronounced irradiation-induced changes whereas the response of the two other enzymes was less significant. Reduction of the protein levels in irradiated samples was important under the XeCl laser coercion and the effects depended on the doses applied. The laser irradiation effects are suggested to be realized by means of conformational changes in the protein molecules and intermolecular association- dissociation processes.

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Svetlana A. Ostrovtsova, Alexander P. Volodenkov, Alexander A. Maskevich, Irina M. Artsukevich, Slavomir S. Anufrik, Alexander F. Makarchikov, Ivan P. Chernikevich, and Vitali I. Stepuro "Effect of laser irradiation on the functional activity of enzymes with different structural complexity", Proc. SPIE 3255, Applications of Ultrashort-Pulse Lasers in Medicine and Biology, (18 May 1998); https://doi.org/10.1117/12.308213

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KEYWORDS

Proteins

Luminescence

Laser irradiation

Molecules

Ultraviolet radiation

Nitrogen lasers

Adaptive optics

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