Development of visible diode-laser/fiber-optic colorimetric system for the simple and inexpensive quantification of a protein

SungHo Kim; Young Soon Noh; Gill Sang Byun; Sung-Keun Jang

doi:10.1117/12.307106

29 April 1998 Development of visible diode-laser/fiber-optic colorimetric system for the simple and inexpensive quantification of a protein

SungHo Kim, Young Soon Noh, Gill Sang Byun, Sung-Keun Jang

Author Affiliations +

Proceedings Volume 3260, Optical Investigations of Cells In Vitro and In Vivo; (1998) https://doi.org/10.1117/12.307106
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

A simple and inexpensive quantitative detection of protein in one droplet solution was achieved by a portable diode-laser- based-optical-fiber absorption spectrometry. A system consisted of a visible diode laser, a photodiode and a pair of optical fibers. The conventional Lowry method was used to measure the blue-colored protein solutions. In this study, one droplet of sample, which filled up the void between the end surfaces of two optical fibers, served as a sample cell. The volume of a droplet was 50 (mu) L, which is 1/100 of the cuvette of UV/VIS spectrometer cell used in Lowry method. Although the required sample volume decreased up to 100 times, the detection range was comparable to that of conventional Lowry method in the range of g/L - mg/L.

Citation Download Citation

SungHo Kim, Young Soon Noh, Gill Sang Byun, and Sung-Keun Jang "Development of visible diode-laser/fiber-optic colorimetric system for the simple and inexpensive quantification of a protein", Proc. SPIE 3260, Optical Investigations of Cells In Vitro and In Vivo, (29 April 1998); https://doi.org/10.1117/12.307106

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