Polarized light  microscopy

Peter Torok; P. D. Higdon; Tony Wilson

doi:10.1117/12.310539

9 June 1998 Polarized light microscopy

Peter Torok, P. D. Higdon, Tony Wilson

Proceedings Volume 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V; (1998) https://doi.org/10.1117/12.310539
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

In this work we discuss the role of polarization in confocal microscopy. The effect of the optical system on light polarization is analyzed in the absence of a specimen. We also present numerical results on confocal microscopes imaging small dielectric scatterers and suggest an optimum pinhole size for crossed linear polars. Finally, imaging of large spherical scatterers and contrast optimization of the optical system is discussed.

Citation Download Citation

Peter Torok, P. D. Higdon, and Tony Wilson "Polarized light microscopy", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); https://doi.org/10.1117/12.310539

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