Video-rate spectral imaging of fluorescence phonomena

Bridget K. Ford; Curtis Earl Volin; Michael R. Descour; John Phillips Garcia; Daniel W. Wilson; Paul D. Maker; Gregory H. Bearman

doi:10.1117/12.328119

16 October 1998 Video-rate spectral imaging of fluorescence phonomena

Bridget K. Ford, Curtis Earl Volin, Michael R. Descour, John Phillips Garcia, Daniel W. Wilson, Paul D. Maker, Gregory H. Bearman

Author Affiliations +

Proceedings Volume 3438, Imaging Spectrometry IV; (1998) https://doi.org/10.1117/12.328119
Event: SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, 1998, San Diego, CA, United States

Abstract

We describe fluorescence spectral-imaging results with the microscope computed-tomography imaging spectrometer ((mu) CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, the (mu) CTIS can be used to image dynamic phenomena involving multiple, spectrally overlapping fluorescence probes. The result presented in this paper consists of proof-of-concept imaging result using two static targets. The first is composed of 6-micrometers fluorescing microspheres and the second consists of rat sinusoid epithelial cells loaded with 0.5-micrometers fluorescing microspheres. Image data were collected in integration times of 16 msec, comparable to video frame rate integration times. The emission spectra were sampled at 10-nm intervals between 420 nm and 710 nm. The smallest spatial sampling interval presented in this paper is 1.7 micrometers .

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Bridget K. Ford, Curtis Earl Volin, Michael R. Descour, John Phillips Garcia, Daniel W. Wilson, Paul D. Maker, and Gregory H. Bearman "Video-rate spectral imaging of fluorescence phonomena", Proc. SPIE 3438, Imaging Spectrometry IV, (16 October 1998); https://doi.org/10.1117/12.328119

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