Multiwavelength videomicrofluorimetric method for a preliminary, fast, and inexpensive screening of the cytoxic properties of new drugs: application to some new marine peptides

Pierre M. Viallet; Emmanuelle Rocchi; Jean Vigo; Jean-Marie Salmon

doi:10.1117/12.351540

9 July 1999 Multiwavelength videomicrofluorimetric method for a preliminary, fast, and inexpensive screening of the cytoxic properties of new drugs: application to some new marine peptides

Pierre M. Viallet, Emmanuelle Rocchi, Jean Vigo, Jean-Marie Salmon

Author Affiliations +

Proceedings Volume 3595, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems; (1999) https://doi.org/10.1117/12.351540
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The discovery of new families of active drugs isolated from natural sources is an appealing avenue to avoid the MDR consequences in cancer treatment. Unfortunately the procedures of isolation and purification of these compounds are generally complex and time-consuming so that only tiny amounts of the new drugs are available for the preliminary biological tests. As a consequence there is a need for reliable, quantitative methods, more informative than the conventional cytotoxicity curve on the potential intracellular targets, and for which only tiny amounts of drugs are necessary. We have recently developed a method of screening based on a triple labeling process of single living cells in culture, involving the simultaneous use of Hoechst 33342 for nuclear staining. Rhodamine 123 for mitochondrial staining and Nile Red for cell delineation. Numerical image analysis was used to study both the dose- effect and the time-effect of well-known cytotoxic drugs such adriamycin, allowing to select the more informative biochemical parameters. The use of this method has recently been extended to new marine peptides such as didemnin B and some laxaphycins.

Citation Download Citation

Pierre M. Viallet, Emmanuelle Rocchi, Jean Vigo, and Jean-Marie Salmon "Multiwavelength videomicrofluorimetric method for a preliminary, fast, and inexpensive screening of the cytoxic properties of new drugs: application to some new marine peptides", Proc. SPIE 3595, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems, (9 July 1999); https://doi.org/10.1117/12.351540

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Oceanography

Tumors

Cancer

Cell death

Image analysis

Proteins

Show All Keywords

Keywords/Phrases

Search In:

Publication Years