Fluorescence detection system for biochips

Arthur H. Rogers

doi:10.1117/12.346742

21 April 1999 Fluorescence detection system for biochips

Arthur H. Rogers

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346742
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

In virtually everyapplicationofbio-medical technology there is at least on step in the process where detection oflight is employed for measurement or quantification. Some cases involve light which is emitted at the molecular level (e.g. chemiluminescence), while others require an external light source and measure changes in the illumination (e.g. fluorescence, absorption, etc.). There are a wide variety of light sources (LEDs, lasers, filament or arc lamps, etc.) available for illumination, as well as a number of photon detectors (photo-multiplier tubes, photodiodes, vidicons, charge coupled devices, etc.). Many bio-medical assays are evolving toward ever greater numbers of active sites per test platform (often hundreds or even thousands), as well as miniaturization ofboth the individual features and the over-all array size. We will present here arguments for using charge coupled devices (CCDs), perhaps cooled or intensified, as "area detectors' and bulbs (filament or arc lamps) as "area illuminators" for such applications. Because of time restrictions, we will limit much of the presentation to simply itemizing features, advantages, and disadvantages. Anyone who has a potential application is encouraged to contact the author for more details and supporting documentation.

Citation Download Citation

Arthur H. Rogers "Fluorescence detection system for biochips", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346742

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KEYWORDS

Charge-coupled devices

Signal detection

Sensors

Fiber optics

Quantum efficiency

Inspection

Optical filters

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