Uptake and distribution of fluorescently labeled cobalamin in neoplastic and healthy breast tissue

Michelle J. Cannon; James M. McGreevy; Joseph A. Holden; Frederick G. West; Charles B. Grissom

doi:10.1117/12.386306

17 May 2000 Uptake and distribution of fluorescently labeled cobalamin in neoplastic and healthy breast tissue

Michelle J. Cannon, James M. McGreevy, Joseph A. Holden, Frederick G. West, Charles B. Grissom

Proceedings Volume 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X; (2000) https://doi.org/10.1117/12.386306
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Fluorescent analogs of cobalamin (vitamin B₁₂) have been developed as diagnostic markers of cancer cells. These compounds are recognized by transcobalamin, a cobalamin transport protein, with high affinity, as shown by surface plasmon resonance. The cellular sequestration and gross distribution of fluorescent cobalamin bioconjugates in breast tissue is being examined by epifluorescence microscopy. The distribution of each compound is being evaluated in proliferative and non-proliferative tissue, i.e. normal tissue and breast carcinoma. The results of preliminary studies suggest that fluorescent analogs of cobalamin may be a useful tool in therapeutic breast operations to define tumor margins and to distinguish neoplastic breast tissue from healthy breast tissue.

Citation Download Citation

Michelle J. Cannon, James M. McGreevy, Joseph A. Holden, Frederick G. West, and Charles B. Grissom "Uptake and distribution of fluorescently labeled cobalamin in neoplastic and healthy breast tissue", Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); https://doi.org/10.1117/12.386306

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available