Image reconstruction in tissuelike turbid media under single and two-photon excitation

Xiasong Gan; N. Dragomir; Min Gu

doi:10.1117/12.379581

15 March 2000 Image reconstruction in tissuelike turbid media under single and two-photon excitation

Xiasong Gan, N. Dragomir, Min Gu

Proceedings Volume 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications; (2000) https://doi.org/10.1117/12.379581
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

In this paper, we present the forward (optical gating) and inverse (reconstruction) approaches to obtain high-resolution imaging in tissue-like media under single-photon (1-p) and two-photon (2-p) excitation. Effective point spread functions (EPSF) for fluorescence microscopic imaging are introduced for fast image modeling and reconstruction. It is demonstrated that a deeper penetration depth can be achieved under 2-p excitation due to the use of a longer illumination wavelength and the non-linear dependence of the fluorescence on excitation intensity. The fundamental difference between 1-p and 2-p fluorescence imaging is that the penetration depth is limited by the degradation in image resolution in 1-p fluorescence, while the penetration depth is limited by the loss in signal strength in 2-p fluorescence imaging. Based on the EPSF that derived in the simulation, image reconstruction using deconvolution methods can partially recover the resolution loss.

Citation Download Citation

Xiasong Gan, N. Dragomir, and Min Gu "Image reconstruction in tissuelike turbid media under single and two-photon excitation", Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); https://doi.org/10.1117/12.379581

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KEYWORDS

Luminescence

Image resolution

Photons

Objectives

Scattering

Image restoration

Point spread functions

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