Modular microinstrumentation for endothelial cell research

Bonnie L. Gray; Abdul I. Barakat; Deborah K. Lieu; Scott D. Collins; Rosemary L. Smith

doi:10.1117/12.379565

15 March 2000 Modular microinstrumentation for endothelial cell research

Bonnie L. Gray, Abdul I. Barakat, Deborah K. Lieu, Scott D. Collins, Rosemary L. Smith

Proceedings Volume 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications; (2000) https://doi.org/10.1117/12.379565
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30 - 300 micrometer wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off- chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m², cuboidal cells grown in 200 micrometer wide microchannels progressively align and elongate in the direction of flow.

Citation Download Citation

Bonnie L. Gray, Abdul I. Barakat, Deborah K. Lieu, Scott D. Collins, and Rosemary L. Smith "Modular microinstrumentation for endothelial cell research", Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); https://doi.org/10.1117/12.379565

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