Imaging of blood antigen distribution on blood cells by thermal lens microscopy

Hiroko Kimura; Kazuya Sekiguchi; Fumiko Nagao; Masahiro Mukaida; Takehiko Kitamori; Tsuguo Sawada

doi:10.1117/12.386331

19 May 2000 Imaging of blood antigen distribution on blood cells by thermal lens microscopy

Hiroko Kimura, Kazuya Sekiguchi, Fumiko Nagao, Masahiro Mukaida, Takehiko Kitamori, Tsuguo Sawada

Proceedings Volume 3916, Biomedical Optoacoustics; (2000) https://doi.org/10.1117/12.386331
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Blood group antigens on a cell were measured by a new microscopic method, i.e. thermal lens microscopy which involves spectrometry using a laser-induced thermal-lens effect. The blood group antigen was immunologically stained using antibody labeled with colloidal gold. Human leukocyte antigens (HLA) on lymphocytes and mononuclear leukocytes were observed by the thermal lens microscope, and Lewis blood group antigens on erythrocytes and polymorphonuclear leukocytes were also observed. The antigen distribution on each cell-surface was imaged using this technique. In spite of convex surface of living cells, colloidal gold was correctly quantified by adjusting the deviation of the focal point of the probe laser by the phase of the signal. In the measurement of leukocyte antigens, antigens of HLA-A, -B, -C loci on the lymphocytes were identified and quantitated by using a single cell. The image of HLA-A, -B, -C antigen distribution on a mononuclear leukocyte was obtained. In the measurement of erythrocyte antigens, a small quantity of Lewis antigens was detected on the cord erythrocytes. Localized small quantities of membrane antigens are better quantitated without extraction or cytolysis. Our thermal lens microscope is a powerful and highly sensitive analytical tool for detecting and quantitating localized antigens in single cells and/or cell-surface-associated molecules.

Citation Download Citation

Hiroko Kimura, Kazuya Sekiguchi, Fumiko Nagao, Masahiro Mukaida, Takehiko Kitamori, and Tsuguo Sawada "Imaging of blood antigen distribution on blood cells by thermal lens microscopy", Proc. SPIE 3916, Biomedical Optoacoustics, (19 May 2000); https://doi.org/10.1117/12.386331

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