Characterization of human neoplastic and normal oral tissues by visible excitation and emission fluorescence spectroscopy

D. Koteeswaran; Nammalver Vengadesan; Prakasa Rao Aruna; K. Muthuvelu; S. Bharghavi; V. S. Gowri; Singaravelu Ganesan

doi:10.1117/12.382745

19 April 2000 Characterization of human neoplastic and normal oral tissues by visible excitation and emission fluorescence spectroscopy

D. Koteeswaran, Nammalver Vengadesan, Prakasa Rao Aruna, K. Muthuvelu, S. Bharghavi, V. S. Gowri, Singaravelu Ganesan

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Proceedings Volume 3917, Optical Biopsy III; (2000) https://doi.org/10.1117/12.382745
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The steady state native fluorescence emission and excitation spectra of human normal and cancerous oral tissues are studied in the visible region. The fluorescence excitation spectrum is recorded for 600 nm emission by scanning the excitation. The excitation spectrum of normal tissues has peaks at 406, 524 and 552 nm, whereas the cancerous tissues have peaks at 406, 513 and 552 nm respectively. The fluorescence emission spectra were also recorded at 405 and 560 nm excitations. The emission spectrum of cancerous tissues has two distinct peaks at 604 and 660 nm. It is also observed that there is a distinct difference between normal and cancerous tissues at 560 nm excitation. The ratio parameter R_{1$ equals (I(subscript 406}/I₅₅₀) is introduced from the excitation spectrum for 600 nm emission and two ratio parameters R₂ equals (I₄₇₀/I₆₀₀) and R₃ equals (I₄₇₀/I₆₆₀) are introduced for the emission spectrum at 405 nm excitation. Among the three ratio parameters the R₁ classifies the normal and cancerous tissues at a specificity and sensitivity of 83 percent and 93 percent respectively. A critical value of 1.8 is suggested for classifying the normal from cancerous tissues.

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D. Koteeswaran, Nammalver Vengadesan, Prakasa Rao Aruna, K. Muthuvelu, S. Bharghavi, V. S. Gowri, and Singaravelu Ganesan "Characterization of human neoplastic and normal oral tissues by visible excitation and emission fluorescence spectroscopy", Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); https://doi.org/10.1117/12.382745

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KEYWORDS

Tissues

Luminescence

Cancer

Fluorescence spectroscopy

Visible radiation

Tumors

Spectroscopy

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