Identification of cancer cells by a combination of FTIR spectroscopy and PET

Gerald Steiner; Tom Richter; Reiner Salzer; Ralf Bergmann; Heike Rodig; Bernd Johannsen

doi:10.1117/12.379587

14 March 2000 Identification of cancer cells by a combination of FTIR spectroscopy and PET

Gerald Steiner, Tom Richter, Reiner Salzer, Ralf Bergmann, Heike Rodig, Bernd Johannsen

Proceedings Volume 3920, Spectral Imaging: Instrumentation, Applications, and Analysis; (2000) https://doi.org/10.1117/12.379587
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

A combination of FTIR spectroscopy and positron emission tomography (PET) is shown to provide new information on tissue. Here we give a first demonstration on the potential of this combination in discriminating tumor tissue from healthy tissue. Examples are taken of cancer grown in muscle tissue in mice. Immediately before thin sections of the cancer tissue were prepared, a radiotracer was injected in the living mouse. Subsequently a native section was immobilized on a CaF₂ window and an autoradiographic image was recorded from that immobilized section. FTIR maps of the thin sections were obtained by using an infrared microscopy equipped with computerized XY stage and MCT detector. Principal component analysis was chosen for chemometric evaluation of the spectra. Evaluated data were reassembled into 2D maps and compared with the corresponding PET image.

Citation Download Citation

Gerald Steiner, Tom Richter, Reiner Salzer, Ralf Bergmann, Heike Rodig, and Bernd Johannsen "Identification of cancer cells by a combination of FTIR spectroscopy and PET", Proc. SPIE 3920, Spectral Imaging: Instrumentation, Applications, and Analysis, (14 March 2000); https://doi.org/10.1117/12.379587

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available