Metabolic cytometry

Sergey N. Krylov; Zheru Zhang; Norman J. Dovichi

doi:10.1117/12.383332

21 April 2000 Metabolic cytometry

Sergey N. Krylov, Zheru Zhang, Norman J. Dovichi

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383332
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Capillary electrophoresis with laser-induced fluorescence detection has the potential to become an important tool in analyzing the chemical contents of single cells. Singe-cell studies are important for understanding the molecular mechanisms of many biological processes, including serious disorders, such as concern and neurodegradative diseases. We developed a new technique, Metabolic Cytometry, that allows us to study complex metabolic pathways in single cells. The essence of the technique is the following. A fluorescently labeled metabolic probe is introduce by incubation into a cell where it is converted to a number of fluorescently labeled metabolites. After incubation the cell is injected into the capillary and lysed. The metabolites are then separated and detected using CE-LIF. If metabolic cytometry is combined with image cytometry, then metabolic activities can be correlated with cellular phenotype or genotype. We applied this powerful approach to correlate glycosylation with the cell's phase in the cell cycle.

Citation Download Citation

Sergey N. Krylov, Zheru Zhang, and Norman J. Dovichi "Metabolic cytometry", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383332

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available