Low-power protein crystallography using polycapillary optics

Huapeng Huang; Carolyn A. MacDonald; Walter M. Gibson; D. C. Carter; J. X. Ho; J. R. Ruble; Igor Yu. Ponomarev

doi:10.1117/12.405885

2 November 2000 Low-power protein crystallography using polycapillary optics

Huapeng Huang, Carolyn A. MacDonald, Walter M. Gibson, D. C. Carter, J. X. Ho, J. R. Ruble, Igor Yu. Ponomarev

Proceedings Volume 4144, Advances in Laboratory-based X-Ray Sources and Optics; (2000) https://doi.org/10.1117/12.405885
Event: International Symposium on Optical Science and Technology, 2000, San Diego, CA, United States

Abstract

This paper describes the design and performance of a low power protein crystallography system using polycapillary optics. The characterization of the source and polycapillary optics are presented. Three optic types: collimating, slightly focusing and strongly focusing optics have been used in low power source-optic combination systems. The source-collimating optic and source- slightly focusing optic systems were used to collect data sets for chicken egg-white Lysozyme with conventional sample oscillation during data collection. The data sets show high quality by analysis with a conventional software package, DENZO. Still diffraction patterns without oscillation have also been obtained by a low power source-strongly focusing optic combination. These patterns have been analyzed by developing software for processing diffraction patterns obtained with strongly convergent X-ray beams. The directions of future work and system improvements are also discussed.

Citation Download Citation

Huapeng Huang, Carolyn A. MacDonald, Walter M. Gibson, D. C. Carter, J. X. Ho, J. R. Ruble, and Igor Yu. Ponomarev "Low-power protein crystallography using polycapillary optics", Proc. SPIE 4144, Advances in Laboratory-based X-Ray Sources and Optics, (2 November 2000); https://doi.org/10.1117/12.405885

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