Response of the fluorescence of tagged proteins on light-assisted modified polymer surfaces

Dan V. Nicolau; Takahisa Taguchi; Hitoshi Suzuki

doi:10.1117/12.411711

29 December 2000 Response of the fluorescence of tagged proteins on light-assisted modified polymer surfaces

Dan V. Nicolau, Takahisa Taguchi, Hitoshi Suzuki

Proceedings Volume 4200, Biochemical and Biomolecular Sensing; (2000) https://doi.org/10.1117/12.411711
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

The fluorescence of fluorescently tagged proteins on functionalized polymer surfaces shifts towards higher wavelengths. Two types of polymers for light-assisted surface manipulation have been used, namely diazo-naphto- quionone/novolak (DNQ/N) resist and poly)tert-butyl- methacrylate). The proteins were either hydrophobicity- attached; covalently linked; or specific protein-protein recognition. We observed that on hydrophilic surfaces the fluorescence is shifted towards lower wavelengths. This parasitic effect has to be taken into account when 'reading' biochips but it can be also used for the 'alignment' of the fluorescence of the fluorescently tagged proteins on the same wavelengths via the manipulation of the properties of the substrate polymer.

Citation Download Citation

Dan V. Nicolau, Takahisa Taguchi, and Hitoshi Suzuki "Response of the fluorescence of tagged proteins on light-assisted modified polymer surfaces", Proc. SPIE 4200, Biochemical and Biomolecular Sensing, (29 December 2000); https://doi.org/10.1117/12.411711

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