Detection of apoptosis by optical coherence tomography (OCT)

Freek J. van der Meer; Dirk J. Faber; Maurice C.G. Aalders; Jop Perree; Ton G. J. M. van Leeuwen

doi:10.1117/12.427888

23 May 2001 Detection of apoptosis by optical coherence tomography (OCT)

Freek J. van der Meer, Dirk J. Faber, Maurice C.G. Aalders, Jop Perree, Ton G. J. M. van Leeuwen

Proceedings Volume 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V; (2001) https://doi.org/10.1117/12.427888
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Apoptosis is the effector of regulated cell death and plays a role in many physiologic and pathologic processes. It is characterized by a highly regulated condensation and fragmentation of the cell nucleus, a large scatterer, and breakup of the entire cell into vesicles, (apoptotic bodies) containing cell organelles and fragments of the nucleus. A two-fold increase in attenuation coefficient ((mu) ) is observed in cell culture after chemical induction of apoptosis. An identical increase in scattering is observed in a tissue culture of porcine carotid artery, in which apoptosis is induced by balloon dilation. These observations are theoretically supported by calculations based on MIE theory. The preliminary results of this study indicate that the apoptotic process may be detected using OCT due to an increase in scattering by the typical disintegration of cellular material. The described increase in scattering may also be detected by other optical techniques.

Citation Download Citation

Freek J. van der Meer, Dirk J. Faber, Maurice C.G. Aalders, Jop Perree, and Ton G. J. M. van Leeuwen "Detection of apoptosis by optical coherence tomography (OCT)", Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); https://doi.org/10.1117/12.427888

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